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Spalletti told reporters that “Locatelli has left” without specifying what injury had led to the Juventus midfielder being forced to depart the Azzurri’s training camp.
Locatelli joins AC Milan captain Davide Calabria, Napoli goalkeeper Alex Meret, and Atalanta defender Rafael Toloi on the treatment table, leaving Spalletti with only 25 of the 29 players he initially called up for the Group C deciders.
Italy are third, three points behind Ukraine who occupy the second automatic qualifying spot in Group C but have played a match more than the European champions.
Spalletti’s team host North Macedonia, who dumped Italy out of the last World Cup in the playoffs, in Rome on Friday night before taking on Ukraine in Leverkusen the following Monday.
Four points from those two matches will give Italy the chance to defend their title at next year’s tournament in Germany.
Locatelli recently signed a long-term deal with Juventus which will keep him at the Serie A club until 2028.
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